A New Paradigm for Individual Subject Language Mapping: Movie-Watching fMRI

BACKGROUND: Functional MRI (fMRI) based on language tasks has been used in presurgical language mapping in patients with lesions in or near putative language areas. However, if patients have difficulty performing the tasks due to neurological deficits, it leads to unreliable or noninterpretable results. In this study, we investigate the feasibility of using a movie-watching fMRI for language mapping. METHODS: A 7-minute movie clip with contrasting speech and nonspeech segments was shown to 22 right-handed healthy subjects. Based on all subjects’ language functional regions-of-interest, 6 language response areas were defined, within which a language response model (LRM) was derived by extracting the main temporal activation profile. Using a leave-one-out procedure, individuals’ language areas were identified as the areas that expressed highly correlated temporal responses with the LRM derived from an independent group of subjects. RESULTS: Compared with an antonym generation task-based fMRI, the movie-watching fMRI generated language maps with more localized activations in the left frontal language area, larger activations in the left temporoparietal language area, and significant activations in their right-hemisphere homologues. Results of 2 brain tumor patients’ movie-watching fMRI using the LRM derived from the healthy subjects indicated its ability to map putative language areas; while their task-based fMRI maps were less robust and noisier. CONCLUSIONS: These results suggest that it is feasible to use this novel “task-free” paradigm as a complementary tool for fMRI language mapping when patients cannot perform the tasks. Its deployment in more neurosurgical patients and validation against gold-standard techniques need further investigation

Focus groups and critical social IS research: How the choice of method can promote emancipation of respondents and researchers.

Critical social research in information systems has been gaining prominence for some time and is increasingly viewed as a valid research approach. One problem with the critical tradition is a lack of empirical research. A contributing factor to this gap in the literature is the lack of agreement on what constitutes appropriate methodologies for critical research. The present paper contributes to this debate by exploring the role that focus group research can play in the critical approach. This paper outlines the main characteristics of critical research with an emphasis on its emancipatory faculties. It then reviews the focus group method from the perspective of critical approach and provides a critical account of two research projects that used focus groups as a method of data collection. The paper presents the argument that focus groups, if designed and executed in light of a critical approach, can contribute to the emancipation of researchers and respondents. This argument is built upon the critical theories of the two most influential theorists in critical social information systems research, namely Ju¨rgen Habermas and Michel Foucault. Critically oriented focus groups have the potential to improve communication and move real discourses closer to Habermas’s ideal speech situation. At the same time, they can contribute to challenging the prevailing orthodoxy and thereby overcome established regimes of truth in the Foucauldian tradition. The paper ends by developing a set of guiding questions that provide a means for researchers to ensure that the emancipatory potential of focus group research can be achieved

Solving the Labour Problem Among Professional Workers in the UK Public Sector: Organisation Change and Performance Management

Public sector reform, Organisational change, Labour process, Performance management, Labour management,

Role of the RAM Network in Cell Polarity and Hyphal Morphogenesis in Candida albicans

RAM (regulation of Ace2p transcription factor and polarized morphogenesis) is a conserved signaling network that regulates polarized morphogenesis in yeast, worms, flies, and humans. To investigate the role of the RAM network in cell polarity and hyphal morphogenesis of Candida albicans, each of the C. albicans RAM genes (CaCBK1, CaMOB2, CaKIC1, CaPAG1, CaHYM1, and CaSOG2) was deleted. All C. albicans RAM mutants exhibited hypersensitivity to cell-wall- or membrane-perturbing agents, exhibiting cell-separation defects, a multinucleate phenotype and loss of cell polarity. Yeast two-hybrid and in vivo functional analyses of CaCbk1p and its activator, CaMob2p, the key factors in the RAM network, demonstrated that the direct interaction between the SMA domain of CaCbk1p and the Mob1/phocein domain of CaMob2p was necessary for hyphal growth of C. albicans. Genome-wide transcription profiling of a Camob2 mutant suggested that the RAM network played a role in serum- and antifungal azoles–induced activation of ergosterol biosynthesis genes, especially those involved in the late steps of ergosterol biosynthesis, and might be associated, at least indirectly, with the Tup1p-Nrg1p pathway. Collectively, these results demonstrate that the RAM network is critically required for hyphal growth as well as normal vegetative growth in C. albicans